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水稻OsWAK1编码一细胞壁相联的受体类似蛋白激酶,对其推断的氨基酸进行预测,其结构组成包括胞外区、跨膜区和胞内激酶区。推断OsWAK1蛋白胞外区与配体识别并结合,是胞外信号传递到胞内的信号转导途径中的重要环节,因此OsWAK1蛋白的胞外区是OsWAK1发挥其生物学功能所必需的。将预测的OsWAK1蛋白胞外区与GFP构建为融合蛋白,通过融合蛋白在烟草细胞中的定位确定OsWAK1蛋白结构上含有预测的胞外区。
Rice OsWAK1 encodes a cell-wall-associated receptor-like protein kinase that predicts its putative amino acids and consists of an extracellular domain, a transmembrane domain, and an intracellular kinase domain. Extrapolation of the extracellular domain of OsWAK1 to ligand recognition and binding is an important part of extracellular signal transduction into the intracellular signal transduction pathway. Therefore, the extracellular domain of OsWAK1 protein is necessary for OsWAK1 to exert its biological functions. The predicted extracellular domain of OsWAK1 and GFP were constructed as fusion proteins and the predicted extracellular region of OsWAK1 protein was confirmed by the localization of the fusion protein in tobacco cells.