Soil alkali-hydrolyzable nitrogen, which is sensitive to N fertilization rate, is one of the indicators of soil nitrogen supplying capacity. Two field experiments were conducted in Dongtai(120°19″ E, 32°52″ N), Jiangsu, China in 2009 and Dafeng(120°28″ E, 33°12″ N), Jiangsu province, China in 2010. Six nitrogen rates(0, 150, 300, 375, 450, and 600 kg ha~(-1)) were used to study the effect of N fertilization rate on soil alkali-hydrolyzable nitrogen content(SAHNC), subtending leaf nitrogen concentration(SLNC), yield, and fiber quality. In both Dongtai and Dafeng experiment station, the highest yield(1709 kg ha~(-1)), best quality(fiber length 30.6 mm, fiber strength 31.6 c N tex~(-1), micronaire 4.82), and highest N agronomic efficiency(2.03 kg kg~(-1)) were achieved at the nitrogen fertilization rate of 375 kg ha~(-1). The dynamics of SAHNC and SLNC could be simulated with a cubic and an exponential function,respectively. The changes in SAHNC were consistent with the changes in SLNC. Optimal average rate(0.276 mg day~(-1)) and duration(51.8 days) of SAHNC rapid decline were similar to the values obtained at the nitrogen rate of 375 kg ha~(-1)at which cotton showed highest fiber yield, quality, and N agronomic efficiency. Thus, the levels and strategies of nitrogen fertilization can affect SAHNC dynamics. The N fertilization rate that optimizes soil alkali-hydrolyzable nitrogen content would optimize the subtending leaf nitrogen concentration and thereby increase the yield and quality of the cotton fiber. Two alkali experiments were conducted in Dongtai (120 ° 19 “E, 32 ° 52” N), Jiangsu, China in 2009 and Dafeng (120 ° 28 “E, 33 ° 12” N), Jiangsu province, China in 2010. Six nitrogen rates (0, 150, 300, 375, 450, and 600 kg ha -1) were used To study the effect of N fertilization rate on soil alkali-hydrolyzable nitrogen content (SAHNC), subtending leaf nitrogen concentration (SLNC), yield, and fiber quality. In both Dongtai and Dafeng experiment station, the highest yield (1709 kg ha ~ -1), best quality (fiber length 30.6 mm, fiber strength 31.6 cN tex -1, micronaire 4.82), and highest N agronomic efficiency (2.03 kg kg -1) were achieved at the nitrogen fertilization The dynamics of SAHNC and SLNC could be simulated with a cubic and an exponential function, respectively. The changes in SAHNC were consistent with the change s in SLNC. Optimal average rate (0.276 mg day -1) and duration (51.8 days) of SAHC rapid decline were similar to the values ​​obtained at the nitrogen rate of 375 kg ha -1 Thus, the levels and strategies of nitrogen fertilization can affect SAHNC dynamics. The N fertilization rate that optimizes soil alkali-hydrolyzable nitrogen content would optimize the subtending leaf nitrogen concentration and thus increase the yield and quality of the cotton fiber.