β-酪蛋白淀粉样纤维沉淀的形成及影响因素

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利用硫磺素T(ThT)荧光分析法和透射电子显微镜检测β-酪蛋白形成淀粉样纤维沉淀(Fibril)的动力学过程,研究了磷脂和硫酸肝素对其Fibril形成的影响.实验结果表明,β-酪蛋白在65℃下,pH值为5.4~9.0的范围内,加热252 h以上,并未形成Fibril,说明β-酪蛋白是一种很好的分子伴侣,在高温、弱酸和弱碱条件下均不形成淀粉样纤维沉淀.甘油磷酸胆碱D6PC和D9PC可以显著地促进β-酪蛋白的Fibril的形成,说明一定条件下蛋白质可能与细胞膜之间存在相互作用而导致其二级构象的转变.硫酸肝素对β-酪蛋白形成Fibril具有促进作用,在炎症组织中,硫酸肝素表达量的增加有可能促进β-酪蛋白形成Fibril,说明乳腺炎与乳腺中的Corpora Amylacea的形成存在一定的联系. Thioflavin T (ThT) fluorescence analysis and transmission electron microscopy were used to determine the kinetics of β-casein formation by Fibril, and the effects of phospholipid and heparan sulfate on Fibril formation were studied. The results showed that β Casein did not form Fibril at 65 ℃ and pH ranged from 5.4 to 9.0 for more than 252 h, indicating that β-casein is a good chaperone. Under the conditions of high temperature, weak acid and weak base , No formation of amyloid fibroids was observed.GPCs D6PC and D9PC can significantly promote the formation of Fibril of β-casein, indicating that the protein may interact with the cell membrane under certain conditions and lead to the secondary conformational transition Heparin sulphate promotes the formation of Fibril by β-casein, and the increased expression of heparin sulfate may promote the formation of Fibril in β-casein in inflammatory tissues, indicating that mastitis is related to the formation of Corpora Amylacea in mammary glands .
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