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目的：研究小鼠Ｂ７修饰的肿瘤细胞疫苗的体内外抗肿瘤作用。方法：应用逆转录病毒载体，将小鼠Ｂ７１和Ｂ７２导入小鼠肝癌细胞Ｈｅｐａｌ６细胞株中，Ｇ４１８筛选后获高表达Ｂ７分子的阳性细胞克隆，经丝裂霉素处理制备成肿瘤细胞疫苗（ＴＣＶ），观察ＴＣＶＢ７体外刺激的脾细胞对不同肿瘤细胞系的抗瘤活性及对不同动物模型的体内免疫保护作用和免疫治疗作用。结果：①ＴＣＶＢ７体外刺激的脾细胞对亲本肝癌细胞和小鼠ＮＫ敏感的Ｙａｃ１细胞的体外细胞毒作用明显增强；②在免疫模型中，经ＴＣＶＢ７免疫的小鼠，能对同系肝癌细胞产生完全的免疫保护作用，并能保持无瘤状态长期存活；③在早期模型中，经ＴＣＶＢ７治疗的小鼠，能部分产生对同系肝癌细胞的免疫治疗作用，肿瘤的生长速度减缓，荷瘤小鼠的生存期延长。肿瘤组织（尤其是肿块包膜下）中淋巴细胞浸润增加；④在晚期模型中，经ＴＣＶＢ７治疗的小鼠，未能产生对同系肝癌细胞的免疫治疗作用。结论：Ｂ７修饰的肿瘤细胞疫苗能明显刺激增强体内外抗肿瘤作用。
Objective: To study the anti-tumor effects of mouse B7-modified tumor cell vaccine in vitro and in vivo. METHODS: The retroviral vector was used to introduce mouse B71 and B72 into mouse hepatocellular carcinoma cell line Hepal6. After G418 selection, a positive cell clone with high expression of B7 was obtained and treated with mitomycin C. The tumor cell vaccine (TCV) was used to observe the anti-tumor activity of TCVB7 stimulated spleen cells against different tumor cell lines and in vivo immune protection and immunotherapy of different animal models. RESULTS: 1The in vitro cytotoxicity of TCVB7 stimulated splenocytes on parental hepatoma cells and mouse NK-sensitive Yac 1 cells was significantly enhanced;2 In the immunization model, mice immunized with TCVB7 were able to produce completeness on syngeneic hepatoma cells. Immune protection, and can maintain long-term survival without tumor; 3 In early models, mice treated with TCVB7 can partially produce immunotherapy effects on syngeneic hepatoma cells, tumor growth rate is slowed, tumor-bearing mice Lifetime extension. Infiltration of lymphocytes in tumor tissue (especially under the tumour subcapsular) increased; 4 In advanced models, mice treated with TCVB7 failed to produce immunotherapeutic effects on syngeneic hepatoma cells. Conclusion: B7-modified tumor cell vaccine can significantly stimulate the enhancement of anti-tumor effects in vivo and in vitro.