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目的探讨2014年广州4株1型登革病毒分离株基因型及来源。方法 4株2014年广州市1型登革病毒分离株提取RNA,逆转录PCR扩增包膜蛋白基因并测序,对其进行系统进化分析,结合流行病学资料进行生物信息学分析。结果 4株2014年1型登革病毒分离株分别来自不同的基因亚型,即基因I型(1株)和基因V型(3株),同广州或广东其他地区2013年分离株高度相似,最初由东南亚或印度输入我国。结论广州市2014年1型登革病毒为由商贸旅游由外地输入到广东地区,并已逐渐本地化,须进一步从蚊媒中证实。
Objective To investigate the genotype and origin of four strains of Dengue virus type 1 in Guangzhou in 2014. Methods Four strains of dengue virus isolated from Guangzhou in 2014 were used to extract RNA. The gene of envelope protein was amplified by reverse transcription polymerase chain reaction (PCR) and sequenced. Phylogenetic analysis was carried out and the bioinformatics analysis was conducted according to the epidemiological data. Results Four strains of Dengue dengue virus type 1 in 2014 were from different genotypes, namely genotype I (1 strain) and genotype V (3 strains), which were highly similar to the 2013 isolates in Guangzhou and other areas of Guangdong. Originally imported into China from Southeast Asia or India. Conclusion Guangzhou 2014 Type 1 dengue virus was imported from the Mainland into Guangdong by commerce and tourism and has gradually been localized and further verified from mosquito vectors.