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用单克隆抗体研究乙型肝炎时HBV的几种抗原在肝组织内的定位,形态学及其与肝组织病变和临床表现的相互关系,国内正在开展中。本文对乙型肝炎慢性活动型46例肝组织用单克隆抗HBcAg抗体,ABC法作组织化学检测,并与多克隆人抗HBcAg抗体检测相比较。单克隆抗体染色背景清晰,非特异性染色低,提高了染色质量。本株单克隆抗体检测之阳性率与多克隆抗体基本一致。HBcAg阳性细胞可表现为胞核型,胞
The use of monoclonal antibodies to study the localization, morphology, and liver tissue lesions and clinical manifestations of several antigens of hepatitis B virus in hepatitis B is currently underway in China. In this article, 46 cases of hepatitis B chronic active liver tissue were detected by histochemistry using monoclonal anti-HBcAg antibody and ABC method, and compared with the detection of polyclonal anti-HBcAg antibody. Monoclonal antibody staining has a clear background, low non-specific staining, and improved staining quality. The monoclonal antibody detection rate of this strain is basically the same as that of the polyclonal antibody. HBcAg positive cells can be expressed as karyotypes.