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目的了解新平县家鼠鼠疫疫源地小肠结肠炎耶尔森菌的分布及病原学特征。方法采集家鼠盲肠、舌头和猪粪便、咽喉粘液以及腹泻患者粪便标本进行小肠结肠炎耶尔森菌的检测与分析。结果检测家鼠盲肠、鼠舌头、猪粪便、猪咽喉粘液物、腹泻患者粪便的标本数分别为722、722、467、237和107份,共分离到61株小肠结肠炎耶尔森菌,总检出率为2.71%,5种标本的检出率分别为2.63%、1.39%、3.85%、2.53%和7.48%,差异有统计学意义(χ2=16.422,P=0.003);分离株包括致病株10株、非致病株51株,有1A、2、3三种生物型和O:3、O:5、O:8等多种血清型,以及六种毒力基因型。猪、鼠、腹泻患者标本检出致病菌株数分别为9、1、0株。结论新平县家鼠鼠疫自然疫源地猪、鼠、腹泻患者是小肠结肠炎耶尔森菌的重要宿主,分离菌株具有遗传多样性,猪、鼠是小肠结肠炎耶尔森菌病的主要传染源。
Objective To understand the distribution and etiological characteristics of Yersinia enterocolitica in the plague foci of Xinping County. Methods The cecum, tongue and pig feces, pharyngopharyngeal mucus and stool samples from diarrhea patients were collected for the detection and analysis of Yersinia enterocolitica. Results The numbers of stool samples from cecum, rat tongue, pig manure, pig throat mucus and diarrhea were 722, 722, 467, 237 and 107, respectively. Totally 61 strains of Yersinia enterocolitica were isolated, The detection rate was 2.71%. The detection rates of 5 kinds of specimens were 2.63%, 1.39%, 3.85%, 2.53% and 7.48% respectively, with significant difference (χ2 = 16.422, P = 0.003) 10 diseased plants and 51 non-diseased plants. There were three biotypes 1A, 2,3 and three serotypes O: 3, O: 5, O: 8 and six virulence genotypes. Pigs, rats, diarrhea specimens of pathogens detected were 9,1,0 strains. Conclusion The pigs, rats and diarrhea patients in the natural foci of domesticating plague in Xinping County are important hosts of Yersinia enterocolitica. The isolated strains have genetic diversity. Pigs and mice are the main infectious diseases of Yersinia enterocolitica source.