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将成型肝炎病毒（ＨＥＶ）中国株结构区第二开放读码框架（ＯＲＦ２）８３７ｂｐｃＤＮＡ片段插入ｐＧＥＸ１λＴ质粒中进行表达。经免疫吸印法证明，该质粒表达的融合蛋白具有识别抗－ＨＥＶ的抗原表位，可用于制备抗－ＨＥＶ检测试剂。
The 837bp cDNA fragment of the second open reading frame (ORF2) of the Chinese strain of hepatitis virus (HEV) was inserted into pGEX1λT plasmid for expression. The results of immunoblotting demonstrated that the fusion protein expressed by this plasmid possesses anti-HEV epitope and can be used to prepare anti-HEV detection reagent.