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目的探讨卵丘细胞分泌的趋化因子5能否预测卵母细胞质量和辅助生育妊娠结局。方法收集75个体外受精周期中获得的562枚卵母细胞的卵泡液、受精液和其后的胚胎培养液,根据卵母细胞的发育命运进行分组:A组为未受精卵母细胞;B组受精后发育为非优质胚胎;C组受精后发育为优质胚胎;D组受精后发育为妊娠胚胎。ELISA方法测定4组卵泡液、受精液和胚胎培养液中CCL5的含量,并将结果进行比较。并分析卵母细胞成熟与CCL5水平的关系。结果 A组卵泡液和受精液中CCL5的含量最低。C组与B组卵泡液和受精液中CCL5的含量比较有显著差异,但与D组比较无明显差别。B、C、D 3组胚胎培养液中CCL5含量比较无明显差异。MII期卵子卵泡液中CCL5含量明显高于MI期和GV期卵子。结论卵丘细胞分泌的CCL5水平与卵母细胞的成熟和妊娠结局相关,可为选择移植胚胎和预测妊娠结局提供新的参考标准。
Objective To investigate whether the chemokine 5 secreted by cumulus cells can predict the quality of oocytes and assist in the outcome of pregnancy. Methods The follicular fluid, fertilized fluid and subsequent embryo culture fluid of 56 in vitro fertilization cycles were collected and grouped according to the developmental fate of oocytes: group A was unfertilized oocytes; group B After fertilization develop into non-high quality embryos; C group after fertilization developed into high quality embryos; D group fertilized after the development of pregnancy embryos. ELISA method was used to determine the content of CCL5 in follicular fluid, fertilized liquid and embryo culture medium, and the results were compared. And analyze the relationship between oocyte maturation and CCL5 levels. Results The content of CCL5 in follicular fluid and fertilized liquid in group A was the lowest. The levels of CCL5 in follicular fluid and fertilized liquid of group C and group B were significantly different, but there was no significant difference compared with group D. There was no significant difference in the content of CCL5 in B, C and D embryo culture medium. The content of CCL5 in MII ovarian follicular fluid was significantly higher than that in MI and GV oocytes. Conclusion The level of CCL5 secreted by cumulus cells is related to the maturation and pregnancy outcome of oocytes. It may provide a new reference standard for selecting embryos and predicting pregnancy outcomes.