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目的探讨茶多酚(tea polyphenols,TP)对60Coγ射线诱导L5178Y细胞凋亡和细胞内活性氧水平(reactive oxygen species,ROS)的作用。方法 L5178Y细胞分为空白对照组、60Coγ射线辐照组、TP组及辐照加TP组共4组,TP组及辐照加TP组培养同时加入茶多酚终浓度分别为25、50、100μg/mL,60Coγ射线辐照组及辐射加TP组细胞给予8 Gy60Coγ射线一次性照射后,采用DCHF-DA探针测定细胞内活性氧(ROS)水平,Annexin V-FITC/PI双染和DNA琼脂糖凝胶电泳检测细胞凋亡。结果 8 Gy60Coγ射线照射后4 h,细胞活力明显降低,出现典型的细胞凋亡,琼脂糖凝胶电泳显示出现凋亡梯状条带,并伴随细胞内ROS水平明显升高,茶多酚能明显改善这些反应,TP处理组与辐照组相比有统计学差异(P<0.01)。结论茶多酚能显著降低60Coγ射线诱导的L5178Y细胞内活性氧产生,降低细胞凋亡率,对γ射线照射有一定的保护作用。
Objective To investigate the effects of tea polyphenols (TP) on the apoptosis and the level of reactive oxygen species (ROS) induced by 60Coγ ray in L5178Y cells. Methods L5178Y cells were divided into blank control group, 60Coγ-ray irradiation group, TP group and radiation plus TP group, 4 groups. TP group and radiation plus TP group were cultured with the same final concentration of tea polyphenol, 25,50,100μg The cells were exposed to 8 Gy of 60Co γ-rays after a single irradiation with 60Coγ-ray and TP plus radiation. DCHF-DA was used to determine the intracellular reactive oxygen species (ROS) levels. Annexin V-FITC / PI double staining and DNA agar Glucose gel electrophoresis was used to detect apoptosis. Results The cell viability was significantly decreased 4 h after irradiation with 8 Gy 60Coγ ray, and typical apoptotic cells appeared. The apoptotic ladder was observed by agarose gel electrophoresis with the increase of intracellular ROS level and the obvious increase of tea polyphenols To improve these responses, there was a significant difference between TP group and irradiation group (P <0.01). Conclusion Tea polyphenols can significantly reduce the reactive oxygen species production of L5178Y cells induced by 60Coγ ray, reduce the apoptosis rate and protect γ ray irradiation.