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目的:建立同时测定天麻中天麻素和3种核苷含量的RP-HPLC方法。方法:采用ZORBAX-SB-C18色谱柱(4.6mm×150 mm,5μm);流动相0.1%磷酸水溶液-甲醇梯度洗脱,柱温25℃,流速0.6 mL·min-1,检测波长270 nm。结果:天麻素、尿苷、鸟苷和腺苷分别在0.286~4.29μg(r=0.999 9),0.028 9~0.433 5μg(r=0.999 7),0.066 8~1.002μg(r=1.000 0),0.135 6~2.034μg(r=1.000 0)与峰面积线性关系良好。结论:该测定方法简便、快速、准确,可以作为天麻的质量控制方法之一。
Objective: To establish a RP-HPLC method for simultaneous determination of gastrodin and three nucleosides in Gastrodia elata Blume. Methods: The mobile phase was eluted with a mobile phase of 0.1% phosphoric acid and methanol with a gradient of 4.6 mL · min-1 on a ZORBAX-SB-C18 column (4.6 mm × 150 mm, 5 μm). The detection wavelength was set at 270 nm. Results: Gastrodin, uridine, guanosine and adenosine showed a significant difference between 0.286 and 4.29 μg (r = 0.999 9), 0.028 9 and 0.433 5 μg (r = 0.999 7), 0.066 8 and 1.002 μg 0.135 6 ~ 2.034μg (r = 1.000 0) and the peak area of a good linear relationship. Conclusion: The method is simple, rapid and accurate and can be used as one of the quality control methods of Gastrodia elata.