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随着各种侵入性检测与治疗手段的推广实施,超广谱抗生素的应用,ICU住院时间延长及多种原因所致的免疫抑制/免疫功能紊乱等因素的存在,使得并发各类真菌感染的几率明显增加,因此需早期诊断和早期治疗。传统的诊断技术,如影像学、标本培养和组织学等在特异性、敏感性和诊断时间上均存在不足,另外半乳甘露聚糖(GM)抗原和葡聚糖的检测不能包括全部真菌病原体及特异性不高。目前迅速发展的分子生物学技术为病原真菌生物学特性的研究提供了可能,DNA扩增的聚合酶链反应(PCR)方法已成为分子生物学的核心技术,被用于多种病原体感染的诊断与菌种鉴别,包括早期诊断深部念珠菌感染。
With the promotion and implementation of various invasive testing and treatment methods, the application of ultra-broad-spectrum antibiotics, prolongation of hospital stay in ICU and immunosuppression / immune dysfunction caused by various reasons, etc., make the occurrence of complicated fungal infections There is a significant increase in the chance of early diagnosis and early treatment. Traditional diagnostic techniques such as imaging, specimen culture and histology are deficient in specificity, sensitivity and diagnostic time. In addition, detection of galactomannan (GM) antigen and dextran can not include all fungal pathogens And specificity is not high. The current rapid development of molecular biology techniques for the study of biological characteristics of pathogenic fungi provide the possibility of DNA amplification of polymerase chain reaction (PCR) method has become the core technology of molecular biology, is used for a variety of pathogen infection diagnosis And species identification, including the early diagnosis of deep Candida infection.