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神经生长因子(NGF)是一种营养神经肽,在感觉和交感神经元的生长、维持和存活中起促进作用。NGF 还是一种神经免疫调节剂,可通过多种途径调节免疫反应。鉴于脾细胞表面存在特异性 NGF 受体,作者分别应用~3H-胸苷(~3H-TdR)掺入法及放射免疫法观察2.5s NGF 对小鼠脾淋巴细胞增殖及其细胞内 cAMP 和 cGMP 含量的影响。结果发现,NGF(4×10~(-7)～4×10~(-8)moL/L)在体外可剂量依赖性地抑制~3H-TdR 掺入到脾淋巴细胞 DNA 中,并能提高细胞内 cAMP 水平,但对 cGMP 无影
Nerve growth factor (NGF) is a nutritional neuropeptide that promotes the growth, maintenance, and survival of sensory and sympathetic neurons. NGF is also a neuroimmunomodulator that regulates the immune response in many ways. In view of the presence of specific NGF receptors on the surface of spleen cells, the authors applied ~3H-TdR incorporation and radioimmunoassay to observe the proliferation of splenic lymphocytes and their intracellular cAMP and cGMP in mice after 2.5 s of NGF. The effect of content. As a result, it was found that NGF (4×10 -7 to 4×10 -8 moL/L) inhibited ~3H-TdR incorporation into spleen lymphocyte DNA in a dose-dependent manner and increased Intracellular cAMP levels, but no effect on cGMP