论文部分内容阅读
采用以甲壳胺为基质合成的CX—1型微载体,进行Vero细胞悬浮培养实验。结果表明,在1mg/ml甲壳胺微载体浓度下,使用RPMI1640培养基,添加10%新生牛血清,37℃培养3h,传代Vero细胞能够快速贴附于微载体上。悬浮培养24h时,细胞浓度达到2.26×105个/ml;培养72h时,细胞浓度达到6.89×105个/ml;培养120h时,细胞浓度达到7.12×105个/ml。电镜观察表明细胞在微载体上长势良好。说明甲壳胺微载体适合Vero细胞高密度悬浮培养
The CX-1 microcarrier synthesized by using chitosan as substrate was used to carry out Vero cell suspension culture experiment. The results showed that the RPMI1640 culture medium supplemented with 10% fetal bovine serum at 37 ℃ for 3 h at 1 mg / ml chitosan microcarrier could rapidly adhere Vero cells to microcarriers. When the cells were cultured in suspension for 24h, the cell concentration reached 2.26 × 105 cells / ml. After cultured for 72h, the cell concentration reached 6.89 × 105 cells / ml. After cultured for 120h, the cell concentration reached 7.12 × 105 cells / ml. Electron microscopy showed that the cells grew well on microcarriers. Chitosan microcarriers suitable for high-density Vero cell suspension culture