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采用“直接观察法”和“分离培养法”对转基因水稻根系微生物群落的初步分析结果表明,导入外源水稻几丁质酶基因(RC24)的转基因水稻根内和根表的微生物群落发生显著变化,转基因水稻根部内生真菌总数显著减少,内生细菌总数显著增加,其内生细菌总数是未转基因亲本对照的10倍左右。采用“直接观察法”测得2个转基因水稻品种有内生真菌的根段率为55.2％和81.1％,而对照为100％,转基因水稻根系真菌和细菌种类与对照存在显著差异,有VA菌根泡囊的根段率显著降低。
Preliminary analysis of the root microbial community in transgenic rice by direct observation and isolation culture showed that the microbial communities in roots and roots of transgenic rice introduced with exogenous rice chitinase gene (RC24) changed significantly , The total number of endophytic fungi in transgenic rice significantly decreased, the total number of endophytic bacteria increased significantly, and the total number of endophytic bacteria was about 10 times that of non-transgenic parents. According to the direct observation method, the root segments of endophytic fungi in two transgenic rice varieties were 55.2% and 81.1%, while the control was 100%. There were significant differences in the root fungi and bacteria species between the transgenic rice and the control, Root vesicle root segment rate was significantly reduced.