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花粉粒有非常坚硬的外壳,为此,国内外注重研究破壳技术,破壳方法不断出现。我们采用高速组织捣碎机进行破壳,取得满意效果。 1.仪器: DS-1高速组织捣碎机,上海标本模型厂制造。 2.实验方法: (1)花粉破壳:取干净的花粉适量,用蒸馏水(1:1)浸泡过夜,取出加蒸馏水适量混合均匀,置高速组织捣碎机内进行破壳粉碎,可得到破壳的花粉乳。操作时要使其转速逐渐地升高或降低,否则,易于损坏机器。效果需要干花粉,将上述花粉乳低温干燥即得。 (2)花粉破壳率检测:称取未经破壳处理的原花粉1g,用蒸馏水配制成10%花粉液,混匀。取此
The pollen grains have a very hard shell. For this reason, research on shell-breaking technology has been focused on at home and abroad, and shell-breaking methods have continued to emerge. We use a high-speed tissue crusher to break shells and achieve satisfactory results. 1. Instruments: DS-1 high-speed tissue grinder, manufactured by Shanghai Specimen Model Factory. 2. Experimental methods: (1) Pollen broken shell: Take appropriate amount of clean pollen, soak it overnight with distilled water (1:1), remove and mix evenly with distilled water, and set it in a high-speed tissue masher to break the shell and crush it. Shell of pollen milk. Operate it to gradually increase or decrease the speed, otherwise, it is easy to damage the machine. The effect requires dried pollen and the above-mentioned pollen milk is dried at a low temperature. (2) Detection of broken shell rate of pollen: Weigh 1 g of raw pollen that has not been shattered, and mix it with distilled water to make a 10% pollen solution. Take this