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运用间歇流水式呼吸仪研究卵形鲳鲹(Trachinotus ovatus L.)在不同温度、盐度下呼吸代谢的变化。结果表明:在19~33℃内,随温度升高,卵形鲳鲹的耗氧率(RO)、排氨率(RN)、代谢率(REO)、排泄率(REN)均不断增大,差异有统计学意义(P<0.05),氨商(QA)与蛋白质供能比(PEP)则下降,差异有统计学意义(P<0.05),并在28~33℃组达到稳定;温度系数(Q10值)在水温由28℃升至33℃时最小,为1.59;肝糖原(LG)及乳酸(LD)均呈下降趋势,且各组LG差异均具统计学意义(P<0.05)。在盐度15~30时,随盐度增大,卵形鲳鲹RO、RN、REO、REN、QA与PEP均呈U型抛物线变化,并在盐度20~25时有最小值;LG与LD变化均不明显。在19~33℃内,卵形鲳鲹代谢水平与水温呈正相关,主要由氧化蛋白质供能,28~33℃的温度条件更利于卵形鲳鲹生长。在盐度15~30内,卵形鲳鲹代谢水平与盐度呈U型抛物线变化,主要由氧化蛋白质供能,盐度20~25利于机体储备蛋白质,即利于生长。
The intermittent flow-through respirator was used to study the changes of respiratory metabolism of Trachinotus ovatus L. at different temperatures and salinities. The results showed that the oxygen consumption rate (RO), ammonia nitrogen (RN), metabolic rate (REO) and excretion rate (REN) increased with the increase of temperature at 19 ~ 33 ℃, (P <0.05). The difference between QA and PEP was significant (P <0.05), and reached the peak at 28 ~ 33 ℃. The temperature coefficient (Q10) was the lowest (1.59) when the water temperature rose from 28 ℃ to 33 ℃. The LG and LD showed a decreasing trend, and the difference between the LG groups was statistically significant (P <0.05) . The RO, RN, REO, REN, QA and PEP showed U-shaped parabola changes with increasing salinity at salinities of 15-30, and had the lowest values at salinities of 20-25. LD changes are not obvious. At 19 ~ 33 ℃, the metabolic level of oyster 鲳 呈 was positively correlated with water temperature, and was mainly supplied by oxidized protein. The temperature of 28 33 ℃ was more conducive to oval 鲳 鲹 growth. Within salinities of 15-30, the metabolic level and salinity of oval 鲳 呈 showed a U-shaped parabola, which was mainly supplied by oxidized protein. The salinity of 20-25 was beneficial to the body to reserve protein, which was beneficial to the growth.