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目的研究糖皮质激素注射小鼠抑郁症样情感行为变化。方法C57/B6雄性小鼠18只随机分为3组,模型组以20 mg/kg剂量每日1次注射皮质酮,持续21 d,另有生理盐水对照组每日注射等剂量生理盐水21 d,空白对照组不做任何处置,观察小鼠体重变化,用敞箱实验及糖水消耗试验测定动物行为。结果抑郁模型组小鼠代表焦虑行为的敞箱试验得分明显降低、反应快感行为的奖赏糖水消耗百分比明显降低。结论糖皮质激素持续注射法可用于制作行为小鼠抑郁模型,以及探究抑郁症行为变化发病机制以及寻找更适合治疗抑郁症的药物。
Objective To study the changes of depression and emotion in mice exposed to glucocorticoid. Methods Eighteen male C57 / B6 mice were randomly divided into three groups. The model group was injected with corticosterone once daily at a dose of 20 mg / kg for 21 days. Another group with saline control was injected with normal saline for 21 days , The blank control group without any treatment, observation of body weight changes in mice, open-box experiments and sugar consumption test animal behavior. Results The mice in the depression model group showed significantly lower scores in the open-box test on anxiety behavior and the percentage of rewards-induced consumption of sugary water in the response to dextrose abuse. CONCLUSION: Continuous injection of glucocorticoid can be used to make behavioral depression model in behavioral mice and explore the pathogenesis of depression behavioral changes and to find more suitable drugs for the treatment of depression.