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赤霉素测定法有溴酸钾滴定法,高锰酸钾目视比色法,荧光法和2,4-二硝基苯肼比色法。其中以溴酸钾法较好,但取样需5mg。荧光法虽能达微克量,但操作不易掌握且误差可达10％。二硝基苯肼法可达半微量水平,但需较多乙醇。作者应用钼兰反应作赤霉素的分光光度测定。赤霉素分子中α-羟基酮可在酸性介质中将12-磷钼杂多酸还原为磷钼杂多兰,其颜色与赤霉素含量成直线关系。钼兰法操作简便,试剂易得,再现性和准确度良好,且能节省乙醇。本文报告此法的适宜条件及制剂中赤霉素含量的测定步骤。
Gibberellin determination of potassium bromate titration method, potassium permanganate visual colorimetry, fluorescence and 2,4-dinitrophenylhydrazine colorimetry. Which potassium bromate method is better, but the sample required 5mg. Fluorescence method can reach the amount of micrograms, but the operation is not easy to grasp and the error up to 10%. Dinitrophenylhydrazine method up to semi-trace levels, but more ethanol. The application of molybdenum blue reaction gibberellin spectrophotometric determination. Gibberellin molecules α-hydroxy ketones in acidic medium will be 12-molybdenum heteropoly acid reduced to phosphomolybdate heteropoly, its color and gibberellin content of a linear relationship. Molybdenum Method is easy to operate, easy to get reagents, reproducible and accurate, and can save ethanol. This article reports the appropriate conditions for this method and the determination of gibberellins in the preparation.