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【目的】了解褐飞虱对吡虫啉产生极高水平抗性后对其主要捕食性天敌黑肩绿盲蝽抗性发展的影响,为协调化学防治与生物防治的关系提供科学依据。【方法】分别用对吡虫啉敏感的褐飞虱种群(SN种群)和极高抗种群(RN种群)连续饲养黑肩绿盲蝽25代以及用吡虫啉对黑肩绿盲蝽连续诱导25代,每隔5代分别测定黑肩绿盲蝽的敏感性和酶活性。【结果】SNCF_(25)种群的敏感性变化不大;RNCF_(25)种群的LC_(50)值是原来的1.53倍;诱导(INCF_(25))种群LC_(50)值是原来的1.66倍。SNC、RNC和INC的黑肩绿盲蝽种群F_5、F_(10)、F_(15)、F_(20)、F_(25)代的酯酶和谷胱甘肽转移酶活性变化与抗性倍数的变化不一致,而多功能氧化酶活性变化与抗性倍数的变化较一致。【结论】黑肩绿盲蝽对吡虫啉的抗性发展较慢,其抗性发展与酯酶和谷胱甘肽转移酶的活性变化无关,与多功能氧化酶活性的变化成正相关。
【Objective】 The objective of this study was to investigate the effects of the planthopper on the development of Apodemus agrarius, a major predatory natural enemy, after producing extremely high level of resistance to imidacloprid, providing a scientific basis for coordinating the relationship between chemical control and biological control. 【Method】 25 generations of Apodemus agrarianus were continuously fed with imidacloprid-sensitive brown planthopper (SN) population and highly resistant population (RN population), respectively. On behalf of the black-shouldered lucorum were measured sensitivity and enzyme activity. 【Result】 The results showed that the sensitivity of SNCF_ (25) population did not change much. The LC_ (50) value of RNCF_ (25) population was 1.53 times of that of original population. The value of LC_ (50) . The changes of esterase and glutathione transferase activities and resistance multiples of F_5, F_ (10), F_ (15), F_ (20) and F_ (25) Of the changes inconsistent, and changes in multi-functional oxidase activity and fold change more consistent. 【Conclusion】 The development of resistance to imidacloprid was slower in Apodemus agrarius. The resistance development was not related to the changes of esterase and glutathione transferase activity, but positively correlated with the changes of multifunctional oxidase activity.