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将带1－2个芽眼的唐菖蒲球茎切块接种到附加1．0mg／LBA的MS基本培养基上可诱导休眠芽萌动、无菌芽转移至附加3．0mg／LBA的培养基上可分化产生丛生芽。丛生芽的继代增殖宜采用附加1．5mg／LBA的培养基生根培养，以MS+NAA0．1－0．5mg／L或MS＋IBA1．5mg／L效果最佳。试管苗移栽存活率可达50％左右。
Inoculation of gladiolus corm cuttings with 1-2 buds onto MS basal medium supplemented with 1.0 mg / L BA induces germination of dormant buds and the aseptic shoots are transferred to medium supplemented with 3.0 mg / L BA for differentiation Generate clusters of buds. The proliferation of cotyledons buds should be rooted culture supplemented with 1.5mg / LBA medium, with MS + NAA0.1-0.5mg / L or MS + IBA1.5mg / L best effect. Test tube seedlings survival rate of transplanting up to 50%.