单核细胞黏附于血管内皮,并迁移、分化、摄脂、转化为泡沫细胞,是动脉粥样硬化形成的早期关键事件,而整联蛋白αL亚基在单核细胞的以上生命过程中起重要的黏附作用。本实验对20只新西兰白兔建立动脉粥样硬化动物模型,收集不同时期模型组与对照组外周血单核细胞,通过实时荧光定量PCR定量检测整联蛋白αL亚基mRNA表达水平,并结合激光共聚焦和流式细胞术对其蛋白表达进行检测。整联蛋白αL从第4周到第12周平均相对表达量分别为1.17313,1.53450,1.93936,同时蛋白表达量也随之升高。结果表明随病理时期的延续,整联蛋白αL的mRNA表达水平和蛋白表达水平均有显著增加,提示整联蛋白αL亚基与动脉粥样硬化病程发展有关。 Monocytes adhere to the vascular endothelium and migrate, differentiate, and lipid into foam cells, an early key event in the development of atherosclerosis, whereas the integrin alpha L subunit plays a role in the life-cycle of monocytes The adhesion effect. In this study, animal models of atherosclerosis were established in 20 New Zealand white rabbits. Peripheral blood mononuclear cells were collected from model and control groups at different stages. Real-time quantitative PCR was used to detect the expression of integrin αL subunit mRNA. Confocal and flow cytometry were used to detect the protein expression. The average relative expression of integrin αL from week 4 to week 12 was 1.17313, 1.53450 and 1.193936, respectively, and protein expression increased at the same time. The results showed that with the continuation of the pathological period, integrin αL mRNA expression and protein expression levels were significantly increased, suggesting that integrin αL subunit and atherosclerosis progression.