论文部分内容阅读
目的探讨CD56分子对急性髓性白血病(AML)之急性单核细胞白血病(M5)细胞生物学及临床特征的影响。方法应用G显带技术及流式细胞仪对45例M5患者分别进行核型及免疫表型检测,并回顾分析其临床资料。结果45例患者中CD56表达阳性17例(37.78%),其中正常核型2例(11.76%),异常核型15例(88.24%),以8号三体和11q23异常易见(分别占23.53%,29.41%);伴CD56抗原表达的患者高表达CD11b,CD14(P<0.01,P<0.05),外周血白细胞计数偏高,骨髓及外周血中原始和幼稚细胞百分比明显升高(P<0.05),较多出现髓外浸润,尤其以淋巴结肿大(P<0.05)和肝脾肿大(P<0.05)明显,伴二系以上受累也明显高于对照组(P<0.05),而中枢神经系统及皮肤浸润与对照组比较差异无统计学意义(P>0.05),同时伴CD56阳性的患者出现较低的完全缓解率及较短的生存期(P<0.05)。结论CD56阳性的M5患者易出现异常核型,高表达CD11b,CD14,预后较差。
Objective To investigate the effects of CD56 on the biological and clinical features of acute myeloid leukemia (M5) in acute myeloid leukemia (AML). Methods K - banding technique and flow cytometry were used to detect the karyotype and immunophenotype of 45 patients with M5. The clinical data were retrospectively analyzed. Results The positive expression rate of CD56 in 45 cases was 37.78%. There were 2 cases with normal karyotype (11.76%) and 15 cases with abnormal karyotype (88.24%). The abnormality of trisomy 8 and 11q23 (23.53% %, 29.41%, respectively). The patients with CD56 antigen expression had high expression of CD11b and CD14 (P <0.01, P <0.05), the peripheral blood leukocyte count was higher, the percentage of primitive and immature cells in bone marrow and peripheral blood were significantly increased (P < (P <0.05) and hepatosplenomegaly (P <0.05), especially those with secondary lineages were also significantly higher than that of the control group (P <0.05), while more extramedullary infiltration was found, especially lymph node enlargement (P <0.05) Central nervous system and skin infiltration were not significantly different from those of the control group (P> 0.05). Patients with CD56 positive had lower complete remission rate and shorter survival time (P <0.05). Conclusion CD56 positive M5 patients prone to abnormal karyotype, high expression of CD11b, CD14, the prognosis is poor.