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应用近红外线光谱技术ＮＩＲＳ对２０名男女优秀自行车运动员在功率车逐级递增负荷过程中，同步测定气体代谢，ＨＲ，ＢＬＡ，功率和股四头肌组织中Ｈｂ／Ｍｂ的光密度变化（ＯＤ）和肌肉毛细血管床的血流量变化（ＢＶ）。结果发现：（１）当运动负荷递增到一定强度，相当于７０－７５％ＶＯ２ｍａｘ时，光密度变化（ＯＤ）显示明显的“转折点”。其功率与通气无氧阈（ＶＴ）呈显著正相关，无明显差异；（２）在递增运动中ＯＤ与Ｂｌａ变化呈显著负相关，ＯＤ“转折点”与Ｂｌａ开始上升点基本同步；（３）ＯＤ“转折点”时工作肌血流量（ＢＶ）达到最大。结果表明，采用近红外线光谱技术无损伤测定局部工作肌群的代谢水平，评定运动员在次极限运动时有氧工作能力是可行的，它为无损伤测定无氧阈提供了一种新的途径。
Near infrared spectroscopy (NIRS) was used to measure the changes of optical density, HR, BLA, power and Hb / Mb OD of 20 male and female elite cyclists simultaneously in the stepwise increase of power car load. And muscle capillary bed blood flow changes (BV). The results showed that: (1) Optical density change (OD) showed a significant “turning point” when the exercise load increased to a certain intensity corresponding to 70-75% VO2max. There was a significant positive correlation between power and ventilatory anaerobic threshold (VT); (2) There was a significant negative correlation between OD and Bla changes during incremental exercise, while OD “turning point” was basically synchronous with Bla onset. (3) OD “turning point” working muscle blood flow (BV) to maximize. The results showed that using near infrared spectroscopy to determine the level of metabolism of local working muscles and assessing aerobic working ability of athletes during sub-extreme exercise provided a new way for non-destructive determination of anaerobic threshold.