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大白菜小孢子培养获得的子叶期胚状体,经粉碎的玻璃碴摩擦后,与农杆菌共培养,在加筛选剂BASTA(含PHOSPHINOTHRICIN5MG/L)的培养基上,再生出数株绿苗.自交留种后,对其后代苗期进行的BASTA抗性鉴定显示,有4株抗此除草剂.SOUTHERN杂交结果显示,抗性植株的基因组中各有一个BAR基因插入位点.对转化株的小孢子进行再培养,后代小孢子植株对BASTA抗性的分离比显示,此转基因株为杂合体“,”Microspore derived embryos of Chinese cabbage (?Brassica campestris? ssp ?pekinensis?)were bruised with crushed glass while coculturing with ?Agrobacterium?,strain C58 The strain harbours vector pDHB321 with CaMV 35S driving bar gene that confers resistance to Basta,a phophinothricin (PPT) based herbicide.There are six green plants regenerated on the selective medium with 5 mg/L PPT out of nearly one thousand treated embryos.Having sprayed a 1%solution of the commercial preparation Basta on one leaf of e...