骨髓移植后的病人往往伴有免疫功能缺陷,特别是在移植后早期(6个月内),为探讨骨髓移植后病人T细胞内钙离子浓度与免疫状态的关系,用PMA+Ionomycin以及CD3分子Cross-Linking刺激后,作免疫抗体标记,进行FACS分析,了解T细胞内钙离子浓度及细胞增殖情况。结果发现骨髓移植后早期(6个月内)病人细胞内钙离子与正常儿童及移植后18个月病人比较显著升高,同时细胞表面标记检查提示CD45RA~+ T细胞减少,CD45RO~+ T细胞增多,DNA合成明显低下,细胞增殖能力降低。骨髓移植后早期细胞内钙离子浓度异常升高与T细胞功能低下的关系尚待进一步探讨。 Patients with bone marrow transplantation are often accompanied by immune dysfunction, especially in the early after transplantation (6 months), to explore the relationship between intracellular calcium concentration and immune status of T cells after bone marrow transplantation, with PMA + Ionomycin and CD3 molecules After Cross-Linking stimulation, immunofluorescent antibody labeling, FACS analysis to understand the T cell calcium concentration and cell proliferation. The results showed that intracellular calcium in patients with early stage of bone marrow transplantation (within 6 months) was significantly higher than that in normal children and 18 months after transplantation. Meanwhile, cell surface marker examination showed that CD45RA ~ + T cells decreased, CD45RO ~ + T cells Increased DNA synthesis was significantly lower, lower cell proliferation. The relationship between abnormally elevated intracellular calcium concentration and low T cell dysfunction after bone marrow transplantation remains to be further explored.