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目的:研究转化生长因子-β1mRNA(TGF-β1mRNA)在胃癌组织中的表达及其与淋巴结转移、术后生存率的关系。方法:用原位杂交法检测66例胃癌切除标本中癌组织和10例癌周组织TGF-β1mRNA表达;结合临床资料分析TGF-β1mRNA与胃癌患者术后生存率的关系。结果:胃癌患者中66.67%在术后5年内死亡。胃癌中TGF-β1mRNA阳性表达率86.36%。TGF-β1mRNA表达阴性或弱阳性者47.50%术后生存5年以上,52.50%5年内死亡,TGF-β1mRNA表达呈中、强阳性者11.54%术后生存5年以上,88.46%5年内死亡(P<0.05)。无淋巴结转移者TGF-β1mRNA阳性表达率75.00%,有淋巴结转移者TGF-β1mRNA阳性表达率97.06%(P<0.05)。TGF-β1mRNA高表达与胃癌的进展呈正相关。结论:TGF-β1可促进胃癌生长,增加胃癌的侵袭能力和淋巴转移。提示对胃癌患者行TGF-β1mRNA检测,可判断胃癌的进展和预后。
Objective: To investigate the expression of transforming growth factor-β1 (TGF-β1) mRNA in gastric carcinoma and its relationship with lymph node metastasis and postoperative survival. Methods: The expression of TGF-β1 mRNA in 66 cases of gastric cancer specimens and 10 cases of peri-cancerous tissues was detected by in situ hybridization. The relationship between TGF-β1 mRNA and the survival rate of gastric cancer patients was analyzed with clinical data. Results: 66.67% of gastric cancer patients died within 5 years after operation. The positive rate of TGF-β1 mRNA in gastric cancer was 86.36%. The expression of TGF-β1mRNA was negative or weakly positive in 47.50% of patients after operation for more than 5 years, and in 52.50% of patients died within 5 years, the expression of TGF-β1mRNA was moderate and strongly positive 11.54% of patients survived more than 5 years and 88.46% of patients died within 5 years <0.05). The positive rate of TGF-β1 mRNA in non-lymph node metastasis was 75.00%. The positive rate of TGF-β1 mRNA in lymph node metastasis was 97.06% (P <0.05). The high expression of TGF-β1mRNA positively correlated with the progress of gastric cancer. Conclusion: TGF-β1 can promote the growth of gastric cancer, increase the invasive ability of gastric cancer and lymphatic metastasis. Tip of gastric cancer patients with TGF-β1mRNA detection, can determine the progress of gastric cancer and prognosis.