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目的探讨浙江地区脑出血患者抵抗素基因+299G>A位点单核苷酸多态性与血浆抵抗素水平的相关性。方法选取344例高血压性基底节出血患者,入院时抽取外周血提取DNA,采用聚合酶反应-限制性片断多态性分析基因型,同时采用ELISA法检测血浆抵抗素浓度,并分析其相关性。结果酶切后可见抵抗素基因+299G>A位点有GG、GA和AA三种基因型。脑出血患者等位基因G和A的频率分别为69.3%和30.7%,基因型GG、GA和AA的频率依次为为49.1%、40.4%和10.5%。脑出血患者各基因型频率符合遗传平衡。GG、GA和AA基因型脑出血患者血浆抵抗素浓度依次为(23.9±7.2)ng/ml、(26.4±7.2)ng/ml和(26.9±9.7)ng/ml。GG基因型患者血浆抵抗素浓度显著低于GA和AA基因型患者(均<0.05),而GA和AA基因型患者血浆抵抗素浓度差异无统计学意义(>0.05)。结论浙江地区脑出血患者抵抗素基因+299G>A位点单核苷酸多态性与血浆抵抗素浓度显著相关,可能参与脑出血炎症反应。
Objective To investigate the association between SNP + 299G> A site SNP and plasma resistin level in patients with cerebral hemorrhage in Zhejiang province. Methods A total of 344 patients with hypertensive basal ganglia hemorrhage were enrolled. Peripheral blood samples were taken from hospital for DNA extraction. Polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP) was used to analyze the genotype. Plasma resistin concentration was measured by ELISA. . Results After digestion, there were three genotypes of GG, GA and AA at + 299G> A locus. The frequencies of allele G and A in patients with ICH were 69.3% and 30.7%, respectively. The frequencies of genotypes GG, GA and AA were 49.1%, 40.4% and 10.5%, respectively. The frequency of genotypes in ICH patients is in line with genetic balance. Plasma resistin concentrations were (23.9 ± 7.2) ng / ml, (26.4 ± 7.2) ng / ml and (26.9 ± 9.7) ng / ml in GG, GA and AA genotypes, respectively. The levels of plasma resistin in patients with GG genotype were significantly lower than those in patients with GA and AA genotypes (all <0.05), while there was no significant difference in the plasma resistin levels between GA and AA genotypes (> 0.05). Conclusion There is a significant correlation between SNP + 299G> A locus and plasma resistin concentration in patients with ICH in Zhejiang province, which may be involved in the inflammatory reaction of ICH.