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通过田间试验和室内分析,研究不同面包小麦品种的产量及其蛋白质含量对氮肥用量的反应。结果表明,4个品种的产量、需肥量和籽粒蛋白质含量都有显著差异。应用SDS—PAGE法结合薄层扫描,得出安农9192和安农91168的HMW—GS组成为1、5+10、7+8,山东187为1、13+16、5+10,豫麦28号为1、7+9、5+10。根据多项式回归,4个品种的最高产量位点依次为8106.0、7255.5、7159.5和6000.0kg/hm~2,其N肥用量分别为337.5,301.5、247.5和321.0kg/hm~2,最高产量的蛋白质含量相应为16.1％、13.2％、11.1％和17.5％。以dY/dN表示各个品种的籽粒形成速率和dP/dN表示其蛋白质生物合成强度,表明4个小麦品种可以区分为三个类型,反映不同墓因型的小麦在氮素利用和转化上的差别。
Field experiments and laboratory analysis were conducted to study the responses of different wheat varieties to the amount of nitrogen fertilizer. The results showed that there were significant differences in yield, fertilizer requirement and grain protein content among the four cultivars. The results of SDS-PAGE and TLC showed that HMW-GS of Annong 9192 and Annong 91168 were 1,5 + 10,7 + 8, Shandong 187 was 1,13 + 16,5 + 10, Yumai Number 28 is 1,7 + 9,5 + 10. According to the polynomial regression, the highest yield loci of the four cultivars were 8106.0, 7255.5, 7159.5 and 6000.0 kg / hm ~ 2, respectively. The N fertilizers were 337.5, 301.5, 247.5 and 321.0 kg / hm ~ The contents correspond to 16.1%, 13.2%, 11.1% and 17.5% respectively. The dY / dN indicates the grain formation rate of each variety and dP / dN indicates the protein biosynthesis intensity, indicating that the four wheat cultivars can be divided into three types, which reflect the differences in nitrogen utilization and transformation of wheat with different tomb types .