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目的:探讨喉鳞状细胞癌组织中微淋巴管密度、位置及增殖情况与喉癌生物学行为和临床病理因素的关系。方法:利用LSAB免疫组化三步法研究淋巴管内皮特异性标记物淋巴管内皮透明质酸受体-1(LYVE-1)在喉鳞状细胞癌组织中的表达,光镜和图像分析观察微淋巴管密度并对淋巴管进行定位;EnVision免疫组化二步法研究Ki67在LYVE-1(+)管腔的表达,评价淋巴管增殖情况。结果:①颈淋巴结转移组瘤巢内LYVE-1(+)管腔密度高于非转移组,差异有统计学意义(P<0.01);T1组瘤巢内LYVE-1(+)管腔密度低于T2、T3、T4组,差异具有统计学意义(均P<0.01),T2组瘤巢内LYVE-1(+)管腔密度低于T3、T4组,差异有统计学意义(均P<0.05),T3组与T4组瘤巢内LYVE-1(+)管腔密度相比差异无统计学意义(P=0.582);瘤巢内LYVE-1(+)管腔密度在声门型组与声门上型组、鳞癌Ⅰ级组与鳞癌Ⅱ级组之间差异无统计学意义(均P>0.05)。②颈淋巴结转移组瘤巢周LYVE-1(+)管腔密度高于非转移组,差异无统计学意义(P>0.05)。③瘤巢内LYVE-1(+)管腔存在Ki67核棕黄色着色,瘤巢周LYVE-1(+)管腔未见这一现象。结论:喉鳞状细胞癌组织LYVE-1标记的瘤巢内淋巴管密度与肿瘤浸润、淋巴结转移正相关;喉鳞状细胞癌肿瘤淋巴管生成主要在瘤巢内,瘤巢内淋巴管在肿瘤的浸润、转移过程中发挥更为重要的作用。
Objective: To investigate the relationship between the lymphatic vessel density, location and proliferation in laryngeal squamous cell carcinoma and the biological behavior and clinicopathological factors. Methods: The expression of LYVE-1, a lymphatic endodermis specific marker, in laryngeal squamous cell carcinoma (LSCC) was studied by LSAB immunohistochemical three-step method. The results of light microscopy and image analysis Lymphatic vessel density and localization of lymphatic vessels; EnVision immunohistochemistry two-step method to study the expression of Ki67 in LYVE-1 (+) lumen and evaluate the proliferation of lymphatic vessels. Results: (1) The density of LYVE-1 (+) lumen in cervical lymph node metastasis group was significantly higher than that in non-metastasis group (P <0.01). LYVE-1 (+) lumen density (P <0.01). The luminal density of LYVE-1 (+) in the tumor nests in T2 group was lower than that in T3 and T4 groups, and the difference was statistically significant (P (P <0.05). There was no significant difference in LYVE-1 (+) lumen density between T3 group and T4 group (P = 0.582). The luminal density of LYVE-1 There was no significant difference between group Ⅰ and group Ⅱ of squamous cell carcinoma (P> 0.05). ② The luminal density of LYVE-1 (+) in the neck lymph node metastasis group was higher than that in the non-metastasis group, the difference was not statistically significant (P> 0.05). There was Ki67 nuclear brown staining in LYVE-1 (+) lumen in the neoplasm, but not in LYVE-1 (+) neoplasm. Conclusion: LYVE-1 labeled laryngeal neoplasia of laryngeal squamous cell carcinoma is positively correlated with tumor infiltration and lymph node metastasis. Lymphangiogenesis of laryngeal squamous cell carcinoma is mainly located in neoplasm, The infiltration, transfer process plays a more important role.