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本实验研究了缺氧、再灌流对培养的新生牛主动脉内皮细胞可溶性粘附蛋白ＧＭＰ－１４０、前列环素（ＰＧＩ_２）和血栓素Ａ_２（ＴＸＡ_２）释放的影响。实验分四组：①正常组，②对照组，③东莨菪碱（Ｓｃｏｐｏｌａｍｉｎｅ）处理组，④过氧化氢酶（Ｃａｔａｌａｓｅ）处理组。研究结果证明对照组细胞缺氧２小时、再灌流３０分钟内皮细胞上清液中ＰＧＩ_２，ＴＸＡ_２、可溶性ＧＭＰ－１４０的释放与正常组相比显著降低。这一结果可促使血管收缩、白细胞粘附、血栓形成等过程。在缺氧的过程中应用Ｓｃｏｐｏｌａｍｉｎｅ能显著增加上述物质的释放，而应用Ｃａｔａｌａｓｅ虽能显著增加ＰＧＩ_２、ＴＸＡ_２的释放，但对可溶性ＧＭＰ－１４０释放的影响与对照组相比无显著差异。由此可见Ｓｃｏｐｏｌａｍｉｎｅ的作用优于Ｃａｔａｌａｓｅ。我们认为Ｓｃｏｐｏｌａｍｉｎｅ是通过其抗脂质过氧化作用保护了血管内皮细胞的分泌功能。
This study investigated the effects of hypoxia and reperfusion on the release of soluble adhesion proteins GMP-140, PGI2 and TXA2 in cultured neonatal bovine aortic endothelial cells. The experiment was divided into four groups: ① normal group, ② control group, ③ scopolamine treatment group, ④ catalase treatment group. The results showed that the release of PGI 2, TXA 2 and soluble GMP-140 in the supernatant of the control group was significantly lower than that of the normal group at 2 hours of hypoxia and 30 minutes of reperfusion. This result can promote vasoconstriction, leukocyte adhesion, thrombosis and other processes. The application of Scopolamine in hypoxia significantly increased the release of these substances, while the application of Catalase can significantly increase the release of PGI_2, TXA_2, but the release of soluble GMP-140 compared with the control group no significant difference. This shows that Scopolamine is better than Catalase. We think Scopolamine protects the secretion function of vascular endothelial cells through its anti-lipid peroxidation.