论文部分内容阅读
目的:8周跑台运动对肥胖大鼠摄食以及血浆和下丘脑相关摄食内分泌指标的影响,初步探讨运动减肥的神经内分泌机制。方法:10周营养性肥胖建模SD大鼠分为对照组(LC)和运动组(LE)。大鼠专用跑台,坡度5%,速度20 m/min,运动时间40 min,进行8周运动后取材。每天观察LC组和LE组大鼠摄食量和体重;测试8周运动后肥胖大鼠血糖等基础指标;放射免疫方法测试血浆和下丘脑ghrelin和obestatin蛋白表达;RT-PCR方法测试下丘脑ghrelin受体GHSR-1a、obestatin假定受体GPR-39基因表达。结果:8周跑台运动后,两组大鼠体重都有增加,只是LE组增加的幅度较小;LE组大鼠摄食量显著下降,血糖等基础指标变化不明显;LE组大鼠血浆ghrelin和obestatin没有影响;下丘脑ghrelin和obestatin显著下降;GHSR-1a基因表达没有影响,而没有发现GPR-39基因表达。结论:Ghrelin可能通过中枢机制影响摄食和能量代谢调节;Obestatin-GPR39通路在能量代谢调节中可能不起作用。
OBJECTIVE: To investigate the effect of treadmill exercise on the feeding of obese rats and the endocrine parameters of plasma and hypothalamus feeding during 8 weeks treadmill exercise, and to explore the neuroendocrine mechanism of exercise-induced weight loss. Methods: Ten-week nutritional obesity model SD rats were divided into control group (LC) and exercise group (LE). Rat treadmill, slope 5%, speed 20 m / min, exercise time 40 min, after 8 weeks of exercise drawn. The daily food intake and body weight of rats in LC group and LE group were measured. The blood glucose and other basic indexes of obese rats after 8 weeks of exercise were measured. The expressions of ghrelin and obestatin in plasma and hypothalamus were detected by radioimmunoassay. The ghrelin of hypothalamus Body GHSR-1a, obestatin assumes receptor GPR-39 gene expression. Results: After 8 weeks treadmill exercise, the body weight of both groups increased, but the increase extent of LE group was smaller. The food intake of LE group decreased significantly, but the changes of blood glucose and other basic indexes were insignificant. The levels of ghrelin And obestatin had no effect; ghrelin and obestatin decreased significantly in the hypothalamus; GHSR-1a gene expression had no effect, but GPR-39 gene expression was not found. CONCLUSIONS: Ghrelin may affect feeding and energy metabolism regulation through the central mechanism; Obestatin-GPR39 pathway may not play a role in energy metabolism regulation.