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A human rectal poorly-differentiated adenocarcinoma cell line HR-8348 is established from thesurgically removed tumor mass of a male adult patient. The cell doubling time is 36.05 h. The mitoticindex is 44.5‰. The cells are all of epithelial type and mostly of polygonal shape. Electron microscopicexamination shows malignant nuclei with enlarged nucleoli; there are abundant microvilli and some se-cretory granules. The plating efficiency in soft agar is 12.9%. In PHA (1μg/ml), the cells show obviousagglutination. Chromosomal analysis reveals a mode of 70 chromosomes per cell, with a marker chromo-some. CEA in cells is detected positively both qualitatively and quantitatively. Hetero-transplantation ofthe cells to nude mice shows that the tumor mass has the same morphology as the original mass fromthe patient, with Alcian blue positive material in the cytoplasm. Repeated detection for mycoplasma con-tamination shows negative.
The cell doubling time is 36.05 h. The mitotic index is 44.5 ‰. The cells are all of epithelial type and mostly of polygonal There are abundant microvilli and some se-cretory granules. The plating efficiency in soft agar is 12.9%. In PHA (1 μg / ml), the cells show obviousagglutination. Chromosomal analysis reveals a mode of 70 chromosomes per cell, with a marker chromo-some. CEA in cells is detected positively both qualitatively and quantitatively. Hetero-transplantation of the cells to nude mice shows that the tumor mass has the same morphology as the original mass from the patient, with Alcian blue positive material in the cytoplasm. Repeated detection for mycoplasma con-tamination shows negative.