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以氯代苯胺(PCA)为选择基质,用驯化技术从降解对二氯苯(pDCB)的富集培养物中得到了以同化PCA为唯一碳源和氮源的混合微生物.将这种固定在填充床反应器中的微生物用于PCA的降解作用研究中.在该反应器里,PCA的生物降解遵循Logistic方程q=qmax/(1+eα-βUv).由方程求出了主要的动力学常数,Ks(半速率常数)和qmax(最大比基质降解速率).于PCA降解的同时,释放氯离子到培养基中.在水力停留时间3h,进水PCA浓度为360mg·L-1情况下,基质的体积降解率达到125mg·L-1·h-1;基质的百分去除率为91%.
Chlorinated aniline (PCA) was used as substrate for selection, and acclimation techniques were used to obtain a mixture of microorganisms that assimilate PCA as the sole carbon source and nitrogen source from the biodegradation culture of pDCB. This microorganism immobilized in a packed bed reactor was used in the study of degradation of PCA. In this reactor, PCA biodegradation follows the Logistic equation q = qmax / (1 + eα-βUv). The main kinetic constants, Ks (half rate constant) and qmax (maximal ratio matrix degradation rate) were found from the equation. At the same time as PCA is degraded, chloride ions are released into the culture medium. Under the conditions of hydraulic retention time of 3h and inlet PCA concentration of 360mg · L-1, the volume degradation rate of the substrate reached 125mg · L-1 · h-1, and the percentage of substrate removal was 91%.