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对采集于广东江门的油棕病叶进行了病原菌分离,通过形态学和ITS序列分析,结果表明此病原菌为小孢拟盘多毛孢菌(Pestalotiopsis microspora),这是在我国属首次发现由小孢拟盘多毛孢菌引起油棕叶斑病。生物学特性研究结果表明:该菌菌丝生长最适宜温度为28~30℃,p H为6.0~8.0;分生孢子萌发最适温度为25~30℃,p H为6.0~9.0。菌丝对碳源的利用效果最高为D-麦芽糖和可溶性淀粉;对氮源的利用效率以蛋白胨最高,明显优于其他氮源,而以可溶性淀粉、D-半乳糖、D-麦芽糖、葡萄糖为碳源,分生孢子的萌发率最高,分别为94.33%、90.00%、88.33%、89.67%,四者差异不显著;以蛋白胨和硝酸钾为氮源,分生孢子萌发率较高,分别为80.33%和74.00%。
Pathogenic bacteria were isolated from the leaves of palm oil collected in Jiangmen, Guangdong. Morphological and ITS sequence analysis indicated that the pathogen was Pestalotiopsis microspora, Pestalotiopsis sp. Caused oil palm leaf spot disease. The results of biological studies showed that the optimal temperature for mycelium growth was 28-30 ℃, p H was 6.0-8.0, conidial germination temperature was 25-30 ℃, and p H was 6.0-9.0. The utilization efficiency of mycelia to carbon source was the highest for D-maltose and soluble starch; the utilization efficiency of nitrogen source was highest in peptone, which was obviously better than other nitrogen sources, but soluble starch, D-galactose, D-maltose and glucose were The germination rates of carbon source and conidia were 94.33%, 90.00%, 88.33% and 89.67%, respectively. There was no significant difference among them. Peptone and potassium nitrate were used as nitrogen source and the germination rates of conidia were 80.33% and 74.00%.