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为发掘抗线虫甜樱桃砧木,以实生钵苗为试材,采用人工接种的方法,研究‘大叶草樱’对北方根结线虫、花生根结线虫、南方根结线虫和爪哇根结线虫的抗性。结果表明:接种后35d,接种北方根结线虫(60株)、花生根结线虫(59株)、南方根结线虫(58株)和爪哇根结线虫(60株)的‘大叶草樱’实生苗中未形成根结的植株(0级)数分别为1、7、2和8,受害等级为1级的植株数分别为59、52、56和52,病情指数分别为19.7、17.6、19.3和17.3。根据抗性评价标准判定‘大叶草樱’高抗北方根结线虫、花生根结线虫、南方根结线虫和爪哇根结线虫。‘大叶草樱’对4种主要根结线虫的抗性均存在免疫和高抗2种基因型,对北方根结线虫、花生根结线虫、南方根结线虫和爪哇根结线虫免疫型植株分别占群体总量的1.7%、11.9%、3.4%和13.3%。由此可见,‘大叶草樱’可判断为优良的抗根结线虫甜樱桃砧木和种质资源。
In order to explore the anti-nematode sweet cherry rootstocks, the seedlings of seedlings were used as test materials and the method of artificial inoculation was used to study the effects of ’Ophiopogon japonicus’ on root-knot nematode, root-knot nematode, root-knot nematode and root knot nematode Resistant. The results showed that 35 days after inoculation, the species “Larix kaempferi” were inoculated with 6 root-knot nematodes (6 strains), 1 root-knot nematode (59 strains), 5 root-knot nematodes (58 strains) The number of plants (0 grade) without root knot in seedling was 1, 7, 2 and 8 respectively, the number of plants with grade 1 injury was 59, 52, 56 and 52 respectively, and the disease index were 19.7 and 17.6, 19.3 and 17.3. According to the resistance evaluation criteria to determine the ’big leaf weed’ highly resistant to root-knot nematode, peanut root knot nematode, root-knot nematode and root-knot nematode. The resistance of Agkistrodon halysii to four major root-knot nematodes were both immune and highly-resistant. The genotypes of the northern root-knot nematode, the root-knot nematode, the root-knot nematode and the root-knot nematode immunized plant Accounting for 1.7%, 11.9%, 3.4% and 13.3% of the total respectively. Thus, the ’big leaf grass cherry’ can be judged as good root-knot nematode sweet cherry rootstocks and germplasm resources.