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目的探讨邯郸地区乙肝病毒感染者基因型分布及其分子流行病学特征。方法收集到邯郸市区及周边各县体检和住院以及本站无偿献血的乙肝病毒感染者血清或血浆,通过ELISA法测定HBsAg、抗-HBs、HBeAg、抗-HBe和抗-HBc。特异性引物法和S基因测序相结合的方法测其基因型。对有临床症状且扩增出基因型的样品进行S基因全长扩增,经测序后采用相关软件进行S基因变异和分子进化研究。结果 306个标本中178份分型成功,其中B基因型2份、C基因型176份,未发现其它基因型。对于血清学模式HBsAg、HBeAg、抗-HBc同时阳性的标本分析成功率为90.48%,显著高于其它血清学模式(P<0.05)。扩增出全长S基因27份,各序列之间同源性最高为99.6%,最低为96.7%。核苷酸差异度P i在381—480 nt最低,为0.00 484。在831—930 nt最高,为0.05664。结论邯郸地区乙肝病毒感染者的优势基因型为C型,病毒变异在156—947核苷酸区段呈非随机分布,在分子进化上和日本毒株同在一个分支。
Objective To investigate genotype distribution and molecular epidemiological characteristics of hepatitis B virus infection in Handan area. Methods The serum or plasma of hepatitis B virus infected persons who were physically and hospitalized in Handan city and surrounding counties and donated by blood donation was collected. The HBsAg, anti-HBs, HBeAg, anti-HBe and anti-HBc were measured by ELISA. Specific primers and S gene sequencing method to determine the genotype. The full-length S gene was amplified from the samples with clinical symptoms and genotypes. After sequencing, the related software was used to study S gene mutation and molecular evolution. Results Of the 306 specimens, 178 were successfully genotyped. Among them, 2 were genotype B and 176 were genotype C, no other genotypes were found. The success rate of serological analysis of HBsAg, HBeAg and anti-HBc positive specimens was 90.48%, which was significantly higher than other serological models (P <0.05). 27 full-length S genes were amplified, the highest homology between the sequences was 99.6% and the lowest was 96.7%. The nucleotide difference P i was the lowest at 381-480 nt, which was 0.00 484. At 831-930 nt, the highest was 0.05664. Conclusion The predominant genotype of hepatitis B virus infection in Handan region is type C, and the variation of virus is nonrandomly distributed in the region of 156-947 nucleotides, which is in the same molecular evolution as the Japanese strain.