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建立酶标仪性能评价和质量控制的方法.方法 配制系列甲基橙溶液,用参考分光光度计对酶标仪进行评价和质控,评价指标为重复性、准确性和线性,并用参考酶标仪与此进行对比测定,比较结果符合率.结果 Spectra Ⅲ型酶标仪重复性误差<1.0%;吸光度为0.000~2.254Abs时,准确性误差<1.0%;有效性线性范围为0.000~2.254Abs,直线回归相关系数为0.9998,回归方程为:Y=0.0085+29.88X,线性误差<1%,与参考酶标仪对比,测定符合率为99%.结论 该评价方法操作简单、结果稳定、有明确的质量判断标准,可以在各级试验室使用.“,” Objective The procedure was established for function evaluates and quality control on the ELISA reader. Mehtods A series of methy1 orange solution was prepared and a reference spectrophotometer was used to check the repeatability the accuracy and the linearity of the instrument. A reference ELISA reader also was used to compare the test results. Results Spectra Ⅲ instrument was checked. The repeatability is better than 1.0%. The inaccuracy is not greater than 1%at 492nm0.000~2.254Abs. the linear range is between 0.000~2.254Absr=0.9998 Y=0.0085+29.88X. The linearity inaccuracy is not greater than 1%. The coincidence rate of results tested by two kind of ELISA reader was 99%. Conclusion The procedure is simple. The result is stable. The determine standard is clear. The method can be used widely.