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目的:建立高效液相色谱法测定华山参中东莨菪内酯含量的方法。方法:色谱柱:Agilent ODS C18柱(250 mm×4.6 mm,5μm),流动相:甲醇-0.3%磷酸溶液(30∶70),柱温:35℃,流速:1.0 mL.min-1,测定波长:344 nm。结果:东莨菪内酯的线性范围:9.84~1476.0 ng(r=0.99998);平均回收率在97.2%~100.7%,RSD为1.4%(n=6)。结论:本方法准确、可靠,重复性好,可用于华山参药材的质量控制。
Objective: To establish a method for the determination of scopoletin in Huashan ginseng by HPLC. Methods: The column was Agilent ODS C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol-0.3% phosphoric acid solution (30:70), the column temperature was 35 ℃, the flow rate was 1.0 mL · min- Wavelength: 344 nm. Results: The linear range of scopoletin was 9.84-1476.0 ng (r = 0.99998). The average recovery ranged from 97.2% to 100.7% with a RSD of 1.4% (n = 6). Conclusion: The method is accurate, reliable and reproducible. It can be used for quality control of Huashan ginseng.