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目的研究卵白蛋白(OVA)引起的Th1/Th2平衡偏移对新生小鼠学习记忆功能和神经发生的影响,及将其应用于神经免疫相关实验研究的可行性。方法将新生C57BJ/6小鼠随机分为OVA处理组和PBS对照组。用卵白蛋白(OVA)塑造Th1/Th2平衡偏移小鼠模型。水迷宫实验检测小鼠海马依赖的学习记忆功能;ELISA法测血清和海马组织的IFN-γ,IL-4以及海马的BDNF水平;免疫荧光组化检测小鼠的神经发生。结果 OVA组小鼠学习记忆能力、神经发生及海马BDNF水平低于PBS组,差异有统计学意义(P<0.05);新生小鼠血清和海马的IFN-γ和IL-4浓度低于对照组(P<0.01),且OVA组小鼠血清和海马中IFN-γ/IL-4比值较对照组明显降低,差异均具有统计学意义(P<0.05)。结论 OVA致敏导致新生小鼠Th1/Th2平衡向Th2偏移,削弱了其学习记忆功能,降低了小鼠的神经发生;OVA不适合作为神经免疫相关研究实验中的空白对照。
Objective To investigate the effect of ovalbumin (OVA) -induced Th1 / Th2 balance on learning and memory function and neurogenesis in newborn mice and its feasibility in neuroimmunological related experimental studies. Methods Newborn C57BJ / 6 mice were randomly divided into OVA treatment group and PBS control group. Th1 / Th2 homeostasis mouse model was modeled with ovalbumin (OVA). The expression of IFN-γ, IL-4 in serum and hippocampus and BDNF in hippocampus were detected by ELISA. The neurogenesis was detected by immunofluorescence in mice. Results The levels of learning and memory, neurogenesis and hippocampal BDNF in OVA group were lower than those in PBS group (P <0.05). The concentrations of IFN-γ and IL-4 in serum and hippocampus of neonatal mice were lower than those in control group (P <0.01), and the ratio of IFN-γ / IL-4 in serum and hippocampus in OVA group was significantly lower than that in control group, the difference was statistically significant (P <0.05). Conclusion OVA sensitization induced a shift of Th1 / Th2 balance to Th2 in neonatal mice, impaired its learning and memory function and decreased neurogenesis in mice. OVA was not suitable as a control for neuroimmunity-related studies.