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克隆并测序了巴西固氮螺菌包括 flbD基因在内的鞭毛基因簇片段.flbD突变株失去运动能力,不能合成极生鞭毛和侧生鞭毛(Fla-Laf-).转入质粒携带的多拷贝flbD基因使该菌株恢复鞭毛和运动能力,但合成侧生鞭毛数目减少,在半固体平板上爬行圈比野生型的大.说明在巴西固氮螺菌中,flbD对极生鞭毛和侧生鞭毛的合成调控起重要作用.
Cloning and sequencing of Azospirillum bradyrhizobium, including flbD gene flagellar gene cluster fragment. FlbD mutants lose exercise capacity and can not synthesize polar flagella and flanks (Fla-Laf-). The transfection of plasmid-derived multi-copy flbD gene restored the flagella and motor ability of the strain, but the number of flagella on the synthesis side decreased and the number of reptiles in the semi-solid plate was larger than that in the wild type. This suggests that flbD plays an important role in the synthesis and regulation of polar flagella and lateral flagella in Azospirilum brasiliensis.