论文部分内容阅读
目的:提取雷丸菌丝蛋白,检测其对H_(22)肝癌细胞荷瘤小鼠的肿瘤抑制作用。方法:随机将50只ICR小鼠分为5组,分别为模型组、环磷酰胺组及雷丸菌丝蛋白高(5 mg/kg)、中(3 mg/kg)、低(1 mg/kg)剂量组,尾静脉给药,1次/d,共8 d。停药次日取血后处死小鼠,计算抑瘤率,以酶联免疫法测定小鼠血清IL-4和IFN-y含量。结果:雷丸菌丝蛋白高、中、低剂量均能有效抑制肿瘤的生长(P<0.05)。高剂量抑制率达36.4%,但低于环磷酰胺43.2%的肿瘤抑制率。雷丸菌丝蛋白在抑制肿瘤生长的同时,能够增加小鼠血清IFN-γ含量,降低血清IL-4含量,并能提高脾脏指数。但对小鼠胸腺、肝脏、心脏、肺脏和肾脏指数无明显影响。结论:雷丸菌丝蛋白对H_(22)肝癌细胞所致实体瘤具有显著抑制作用,并能在一定程度上增强小鼠免疫功能。
OBJECTIVE: To extract mycelium protein from Lei beam and detect its tumor inhibitory effect on H22 hepatoma-bearing mice. Methods: Fifty ICR mice were randomly divided into five groups: model group, cyclophosphamide group and Leihuan mycelium protein high (5 mg / kg), medium (3 mg / kg) and low (1 mg / kg) dose group, tail vein administration, 1 time / d, a total of 8 d. The mice were sacrificed on the next day after drug withdrawal, and the tumor inhibition rate was calculated. The levels of IL-4 and IFN-γ in serum were determined by enzyme-linked immunosorbent assay. Results: The mycelia of Lei Jun, high, medium and low doses can effectively inhibit tumor growth (P <0.05). High-dose inhibition rate of 36.4%, but lower than 43.2% of cyclophosphamide tumor inhibition rate. Lei mushroom mycelium protein in inhibiting tumor growth at the same time, can increase serum IFN-γ levels, lower serum IL-4 content, and can improve the spleen index. But had no significant effect on mouse thymus, liver, heart, lung and kidney index. Conclusion: Myrica sinensis mycelium protein has a significant inhibitory effect on solid tumors caused by H_ (22) hepatocellular carcinoma cells, and can enhance the immune function of mice to some extent.