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目的研究纤维蛋白原(Fbg)等危险因素在冠心病(CHD)中的作用。方法对1017例CHD组和470例对照者组分别进行血浆Fbg浓度及其相关基因((HaeⅢ多态性)测定分析。外周血白细胞抽提脱氧核糖核苷酸(DNA),采用酚/氯仿方法;多聚酶链反应(PCR)加酶切技术检测Fbg(HaeⅢ多态性。血浆Fbg浓度(200～400mg/dl)测定采用凝血酶法。血脂测定采用标准酶法。高血压病、糖尿病入选标准:①既往有明确高血压病、糖尿病病史者;②本次入院确诊者。每日吸烟1支以上超过1年定为吸烟者。所有数据采用SPSS11.0统计软件进行统计分析。结果(1)年龄、吸烟、高血压、糖尿病、血清胆固醇、三酰甘油和血浆Fbg水平是CHD危险因素;(2)Pearson相关分析显示年龄、高血压、糖尿病、血清胆固醇、高密度脂蛋白胆固醇、Fbg(HaeⅢ多态性和吸烟对血浆Fbg水平有明显影响。结论除已公认的CHD危险因素外,血浆Fbg浓度增高可能也是CHD的独立危险因素之一,并明显受其基因(HaeⅢ多态性和吸烟等诸多因素的影响。
Objective To investigate the role of fibrinogen (Fbg) and other risk factors in coronary heart disease (CHD). Methods The plasma concentrations of Fbg and its related genes (Hae Ⅲ polymorphism) were determined in 1017 CHD patients and 470 control subjects, respectively.Detecting the deoxyribonucleotides (DNA) in peripheral blood leukocytes by phenol / chloroform method ; Polymerase chain reaction (PCR) plus enzyme digestion technology to detect Fbg (Hae Ⅲ polymorphism. Plasma Fbg concentration (200 ~ 400mg / dl) was determined by thrombin method. Blood lipids using standard enzyme method. Hypertension, Diabetes inclusion criteria: ① previous history of patients with definite hypertension and diabetes mellitus; ② the hospitalized patients were diagnosed as having smoked more than 1 cigarette daily for more than 1 year.All the data were analyzed by SPSS11.0 statistical software.Results (1) Age (2) Pearson correlation analysis showed that age, hypertension, diabetes, serum cholesterol, high density lipoprotein cholesterol, Fbg (Hae Ⅲ multi Status and smoking had a significant effect on plasma Fbg levels.Conclusion In addition to the recognized risk factors for CHD, increased plasma Fbg levels may also be an independent risk factor for CHD, and significantly affected by its gene (Hae Ⅲ Polymorphism and smoking and many other factors.