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目的研究不同产地不同生长年限西洋参中氨基酸含量差异。方法采用微波消解法水解西洋参粉得到氨基酸,以异硫氰酸苯酯(PITC)为柱前衍生试剂,采用高效液相(HPLC)法进行氨基酸含量测定。结果 3年生西洋参较其它年份西洋参中氨基酸含量少,3年后西洋参中各氨基酸含量逐年增加;不同产地西洋参中各氨基酸含量存在差异。结论不同年限西洋参中氨基酸呈“V”字型增长;长白山地区不同产地西洋参中各氨基酸含量不同,集安产地西洋参中各氨基酸含量最多,通化产地西洋参各氨基酸含量最低。
Aim To study the differences of amino acid content in American ginseng from different habitats in different growing years. Methods Amino acids were hydrolyzed by American ginseng powder by microwave digestion. Amino acids were determined by high performance liquid chromatography (HPLC) using phenylisothiocyanate (PITC) as pre-column derivatization reagent. Results Compared with other years, the 3-year-old American ginseng had less amino acids, and the content of amino acids in American ginseng increased year by year after 3 years. The content of amino acids in American ginseng from different areas was different. CONCLUSIONS: The amino acids in American ginseng grew in a “V” shape over different years. The contents of amino acids in American ginseng from different habitats in Changbai Mountain were different. The contents of amino acids in American ginseng in Ji’an were the highest, while those in American ginseng in Tonghua were the lowest.