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本文用邻苯二甲醛及巯基乙醇作试剂与山黧豆毒素的水解产物DAP在pH8形成强荧光产物,在0.01—1μg/ml呈线性关系,其它氨基酸无干扰,从而可测定ODAP,回收率在97％以上。
In this paper, phthalaldehyde and mercaptoethanol as the reagent and the hydrolysis of diazoxide toxin DAP at pH8 form a strong fluorescent product, linear in the 0.01-1μg / ml, no interference with other amino acids, which can be measured ODAP, the recovery rate of More than 97%.