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研究了超高效液相色谱法同时测定面制品中的富马酸二甲酯、山梨酸、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、对羟基苯甲酸丁酯和对羟基苯甲酸戊酯分析方法,以Waters ACQUITY UPLCTMBEH C18(50 mm×2.1mm,1.7μm)柱为分离柱,以流动相为0.1%甲酸水溶液-甲醇进行梯度洗脱,采用二极管阵列在210 nm和260 nm进行检测,整个分离过程在4.50 min内完成,6种组分在0.312 5~10μg/m L浓度范围内的线性关系良好,方法检出限分别为0.019 5,0.002 2,0.019 6,0.026 1,0.046 4和0.360μg/g,样品加标回收率为81.36%~103.10%;相对标准偏差为0.41%~8.40%,该方法简单,分离效果好,分析时间短,能够满足大量日常面制品中防霉剂和防腐剂的检测需要。
The determination of dimethyl fumarate, sorbic acid, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate, butyl p-hydroxybenzoate and p-hydroxybenzoate in the flour products by ultra performance liquid chromatography Ester analysis was performed on a Waters ACQUITY UPLC TMBEH C18 (50 mm × 2.1 mm, 1.7 μm) column with a mobile phase of 0.1% formic acid in methanol - gradient elution at 210 nm and 260 nm using a diode array , The whole separation process was completed within 4.50 min. The linearity of the six components in the concentration range of 0.312 5 ~ 10 μg / mL was good. The detection limits were 0.019 5,0.002 2,0.019 6,0.026 1,0.046 4 And 0.360 μg / g respectively. The recoveries of spiked samples were 81.36% -103.10%. The relative standard deviations were 0.41% -8.40%. The method was simple, the separation was effective and the analysis time was short. And preservative testing needs.