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应用聚合酶链式反应－单链构象多态性分析（ＰＣＫ－ＳＳＣＰ）法检测３０例乳腺癌组织中Ｐ基因的５、６、７３个外显子。结果显示，１５例有异常电泳带，表明这些病例相应的ＤＮＡ片段发生突变。结合临床分析发现：在（１）Ⅱ期７／１４例、Ⅲ期６／１１例、Ⅳ期２／２例，（２）浸润性导管癌１３／２６例、乳头状腺癌１／１例、导管内癌１／１例，（３）腋窝淋巴结阳性１１／１８例、腋窝淋巴结阴性４／１２例中发现Ｐ基因突变。提示Ｐ基因突变与临床分期及病理改变有关，有Ｐ５３基因突变的原发病灶具有较强的侵袭能力，易复发和转移。
Polymerase chain reaction-single strand conformation polymorphism analysis (PCK-SSCP) was used to detect 5, 6, and 73 exons of P gene in 30 cases of breast cancer. The results showed that 15 cases had abnormal electrophoresis bands, indicating that the corresponding DNA fragments of these cases were mutated. Combined with clinical analysis found: in (1) 7/14 cases of stage II, 6/11 cases of stage III, 2/2 cases of stage IV, (2) 13/26 cases of invasive ductal carcinoma, 1/1 case of papillary adenocarcinoma 1/1 case of intraductal carcinoma, (3) 11/18 cases with positive axillary lymph nodes and 4/12 cases with negative axillary nodes. It is suggested that P gene mutations are related to clinical stages and pathological changes. The primary lesions with P53 gene mutations have strong invasive ability and are easy to relapse and metastasis.