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为探讨氮肥对水稻(Oryza sativa)穗发育的调控作用,使用高通量测序技术检测氮肥处理前后水稻叶片和幼穗组织中转录组的变化,并从中筛选到大量差异表达基因。这些基因的功能涉及转录调控、激素代谢和信号转导、物质代谢和转运、胁迫响应、信号转导(受体)和蛋白质降解等。同时对目前克隆得到的穗发育相关基因进行分析,发现在氮素穗肥的作用下,部分重要功能基因的表达量发生了明显变化,其中一些基因还参与调控水稻株高、抽穗期、分蘖和结实率等性状。对这些差异表达基因的功能研究有助于揭示氮素穗肥调控水稻每穗颖花数的分子机制。
In order to investigate the regulatory effect of nitrogen fertilizer on the development of rice (Oryza sativa) spikes, high-throughput sequencing was used to detect the changes of transcriptome in rice leaves and young panicles before and after nitrogen application. A large number of differentially expressed genes were screened out. The function of these genes involves transcriptional regulation, hormone metabolism and signal transduction, substance metabolism and transport, stress response, signal transduction (receptor) and protein degradation. At the same time, we analyzed the cloned ear development related genes and found that the expression of some important functional genes changed obviously under the action of nitrogen panicle fertilization. Some of them also participated in the regulation of plant height, heading date, tillering and Seed set rate and other traits. The study of the function of these differentially expressed genes is helpful to reveal the molecular mechanism of nitrogenous fertilizer on the regulation of spikelet number per panicle in rice.