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AIM:To construct a recombinant E.coli strain that wouldhighly express the proinflammatory outer membraneprotein of human Helicobacter pylori(H pylori).METHODS:The oipA DNA was amplified by PCR,insertedinto pET-32a,and transformed into Top10 E.coli strain.This recombinant plasmid of Top10 was sent out fornucleotide sequence analysis.Finally this sequenceAF479754 was compared with HP0638 and JHP0581.RESULTS:The sequence of the aim gene was obtained.Ithad 924 base pairs.The identity was 95.32% againstHP0638,95.02% against JHP0581,which was higher thanthe identity between HP0638 and JHP0581.CONCLUSION:Although the aim gene was obtained,butit was different from the published sequence of GenBank.Itis not clear what makes this difference.Maybe it is becausedifferent strain was used or because there were somevariations.So more researches are required to prove it.Chen DR,Huang AL,Tao XH,Wang PL,Jiang Z.Cloning andsequence analysis of gene oipA encoding an outer membraneprotein of human Helicobacter pylori.World J Gastroenterol2004;10(21):3205-3207http://www.wjgnet.com/1007-9327/10/3205.asp
AIM: To construct a recombinant E. coli strain that would highly express the proinflammatory outer membrane protein of human Helicobacter pylori (METHODS: The oipA DNA was amplified by PCR, inserted in pET-32a, and transformed into Top10 E. coli strain. This recombinant plasmid of Top10 was sent out for nucleotide sequence analysis. Finaally this sequence AF479754 was compared with HP0638 and JHP0581.RESULTS: The sequence of the aim gene was was.Ithad 924 base pairs. The identity was 95.32% against HP0638, 95.02% against JHP0581, which was higher thanthe identity between HP0638 and JHP0581.CONCLUSION: Although the aim gene was obtained, butit was different from the published sequence of GenBank. Is not clear what makes this difference. Maybe it is becausedifferent strain was used or because there were somevariations. So more researches are required to prove it. Chen DR, Huang AL, Tao XH, Wang PL, Jiang Z. Cloning and sequence analysis of gene oipA encoding an outer membrane protein of human Helicoba cter pylori. World J Gastroenterol 2004; 10 (21): 3205-3207http: //www.wjgnet.com/1007-9327/10/3205.asp