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大豆叶茸毛形态对抗虫性、耐旱性等均有重要作用。本研究利用2个重组自交系群体NJRIKY(KY)和NJRIXG(XG)进行叶面茸毛密度和长度的遗传与QTL定位分析。结果表明,2个性状在2个群体中均有大幅度变异,存在不同程度的超亲分离,两者有极显著负相关(r=–0.49和–0.62),叶面茸毛密度的遗传率(75.7%～76.8%)高于叶面茸毛长度的遗传率(45.2%～62.9%);检测到2个叶面茸毛密度主效QTL(XG群体的PD1-1和KY群体的PD12-1,表型贡献率分别达20.7%和21.7%);两群体叶面茸毛密度遗传构成中加性QTL贡献率占20.7%～36.2%,互作QTL只占0～1.4%,而未定位到的微效QTL所占份额很大,为38.1%～56.1%,是以往只用定位程序而未注意遗传构成解析所没有发现的特点;未在KY中检测到叶面茸毛长度加性QTL,互作QTL贡献率也仅4.2%,而微效QTL贡献率达58.7%;但在XG中叶面茸毛长度加性QTL PL1-1和PL12-1贡献率分别达18.3%和22.5%,占主要成分,互作QTL和微效QTL贡献均较小,说明该性状两群体的遗传构成有很大差异。大豆叶面茸毛密度和长度的遗传涉及多个效应不同的基因/QTL。
Soybean leaf hair morphology on insect resistance, drought resistance, etc. have an important role. In this study, two recombinant inbred lines NJRIKY (KY) and NJRIXG (XG) were used for genetic analysis and QTL mapping of leaf density and length. The results showed that there were significant variations in the two traits among the two populations, with different degrees of super-pro-segregation. There was a significant negative correlation between the two traits (r = -0.49 and -0.62). The heritability of leaf hair density 75.7% -76.8%) were higher than that of the leaf length (45.2% -62.9%). Two major QTLs for leaf hair density were detected (PD1-1 in XG population and PD12-1 in KY population Type contribution rate of 20.7% -36.2% in the genetic constitution of the two groups, while the QTL for interaction accounted for only 0-1.4%, but the undifferentiated micro-effect QTL accounted for 38.1% ~ 56.1% of the total, accounting for a large number of QTLs that were not found in genetic analysis using only the locating program in the past; Rate of only 4.2%, while the contribution rate of micro-effect QTL reached 58.7%. However, the contribution rates of QTL for PLT1 and PL12-1 in XG were 18.3% and 22.5% respectively, accounting for the major components The contribution of QTL and micro-QTLs were small, indicating that the genetic composition of the two populations of the traits are very different. Inheritance of leaf density and length in soybean involves multiple genes / QTLs with different effects.